HUBEI AGRICULTURAL SCIENCES ›› 2019, Vol. 58 ›› Issue (7): 92-95.doi: 10.14088/j.cnki.issn0439-8114.2019.07.021

• Animal Husbandry & Veterinary Medicine • Previous Articles     Next Articles

Synthesis and identification of enramycin artificial antigen

YE Jian-qiang1,2, YU Hua3, KANG Run-min1,2, YAN Yu-bao3, XIE Jing1,2, HU Juan3, LIAO Dang-jin1,2, ZHOU Min-jiang3, XIAO Lu1,2, CUI Peng-bo3, CAO Ye1,2, YE Yong-gang1,2, YU Ji-feng1,2, LI Xing-yu1,2, LIN Yi1,2, PAN Meng1,2, WEI Yong1,2, DAI Zhuo-jian1,2   

  1. 1.Sichuan Animal Science Academy,Chengdu 610066,China;
    2.Key Laboratory of Animal Genetics and Breeding of Sichuan Province,Chengdu 610066,China;
    3.Sichuan Entry-Exit Inspection and Quarantine Bureau,Chengdu 610041,China
  • Received:2018-10-15 Online:2019-04-10 Published:2019-12-03

Abstract: In order to establish an enzyme-linked immunosorbent assay (ELISA) for examing residual Enramycin (Er) in food. The antibody with high titer and specificity must be firstly prepared. So it was necessary for antibody production to conjugate the hapten (Er) with a carrier protein to synthesize immunogent(antigen). Enramycin was coupled with carrier protein bovine serum albumin (BSA) to obtain immunogent (Er-BSA) by glutaraldehyde (GA) method and with ovalnumin (OVA) to obtain coating antigen(Er-OVA) by GA respectively. The successful linkage of Er-BSA and Er-OVA were identified by UV spectrophotometry. The UV spectral characterization demonstrated that Er-BSA and Er-OVA were successfully synthesized at 270~280 nm. The coupling ratios between Er and BSA or OVA were determined to be 26∶1, respectively.

Key words: Enramycin, synthesis of antigen, glutaraldehyde method

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