Changes of polyamines produced by PGPR strains DM under stress conditions

doi:10.14088/j.cnki.issn0439-8114.2019.13.009

Abstract

Abstract: PGPR strains DM（Enterobacter cloacae） was cultured in the medium with different pH and NaCl concentration, and the polyamines in the supernatant of bacteria were qualitatively analyzed by high performance liquid chromatography （HPLC）. The results showed that the concentrations of putrescine, spermidine and spermidine were increased rapidly with NaCl adding, maximum value reached at the culture medium with 150 mmol/L NaCl, and then decreased. The concentration of three polyamines was the highest at the medium of pH 8, then the concentration of polyamines decreased when pH was less than 8, and also the concentration of polyamines decreased when pH was more than 8. The concentration of polyamines produced by DM bacteria changed under the salt, acid and alkali stress, this conclusion suggested that DM bacteria may respond to abiotic stress by regulating the concentration of polyamines.

Key words: plant growth-promoting bacteria PGPR, Enterobacter cloacae, stress, high performance liquid chromatography （HPLC）, polyamine

CLC Number:

Q936

LIU Xiu, ZHENG Yi-ling, LI Yan-qiu, FAN Ya-jun. Changes of polyamines produced by PGPR strains DM under stress conditions[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(13): 44-47.

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