HUBEI AGRICULTURAL SCIENCES ›› 2019, Vol. 58 ›› Issue (17): 129-131.doi: 10.14088/j.cnki.issn0439-8114.2019.17.034

• Biological Engineering • Previous Articles     Next Articles

Establishment of RT-PCR method for the detection of equine influenza virus H3N8

LIN Zhi-xiong1,2, YU Hai-qiong1,2, WANG Ying1,2, ZHAI Jian-xin3, ZHANG Li3   

  1. 1.Guangdong Provincial Key Laboratory of Import and Export Technical Measures of Animal,Plant and Food,Guangzhou 510623,China;
    2.Guangzhou Custom Inspection and Quarantine Technical Center,Guangzhou 510623,China;
    3.Shenzhen Aodong Inspection & Testing Technology Co.,Ltd.,Shenzhen 518000,Guandong,China;
  • Received:2019-04-30 Published:2019-11-14

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Abstract: By comparing the H3N8 sequence of Equine influenza virus （EIV） from NCBI, highly specific primers and probes were designed for the highly conserved region of HA and NA gene sequences, and the program was optimized to establish a RT-PCR method for the detection of EIV H3N8. The results showed that the minimum detection concentration was 3.36×102 copies/μL. With high specificity, only EIV H3N8 samples were positive. The positive detection rate of 58 clinical samples was 12.07%, 2.3 times that of conventional PCR, and 100% consistent with virus isolation.

Key words: Equine influenza virus（EIV）, H3N8 subtype, RT-PCR

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