Analysis of carotenoids chlorophyⅡ and in cassava tissue culture seedlings

doi:10.14088/j.cnki.issn0439-8114.2020.04.028

HUBEI AGRICULTURAL SCIENCES ›› 2020, Vol. 59 ›› Issue (4): 136-140.doi: 10.14088/j.cnki.issn0439-8114.2020.04.028

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Analysis of carotenoids chlorophyⅡ and in cassava tissue culture seedlings

LUO Xiu-qin¹, WEI Zhuo-wen¹, YANG Long^1,2, XUE Jing-jing¹

1.Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences (CATAS)/Key Laboratory of Conservation and Utilization of Cassava Genetic Resources,Ministry of Agriculture and Rural Affairs,Haikou 571101,China;
2.Subtropical Crop Institute of Guizhou Agricultural Science Academy,Xingyi 562400,Guizhou,China

Received:2019-04-28 Online:2020-02-25 Published:2020-05-13

Abstract

Abstract: Ultraviolet-visible spectrophotometer was used to detect the content of chlorophyⅡ and carotenoids in TM60444 and SC9 tissues culture seedings. Meanwhile, the relative genes of carotenoids biosynthesis were analyzed. The results showed that there is nearly no difference between two stains and the contents of chlorophyll and total carotene in two seedings: leaf>stem>root; and the content of carotenoids: leaf>stem, while the stem is approximately the root.

Key words: cassava Manihot esculenta Crantz, carotenoid, RT-PCR, UV spectrophotometer

CLC Number:

S533

LUO Xiu-qin, WEI Zhuo-wen, YANG Long, XUE Jing-jing. Analysis of carotenoids chlorophyⅡ and in cassava tissue culture seedlings[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(4): 136-140.

References

[1] 陈冠喜,李开绵,叶剑秋,等.6个木薯品种生长发育及产量性状的初步研究[J].热带农业科学,2009,29(6):26-29.
[2] 张振文,李开绵,叶剑秋,等.木薯光合作用特性研究[J].云南大学学报(自然科学版),2007,29(6):628-632.
[3] LI K M,ZHU W L,ZENG K,et al.Proteome characterization of cassava (Manihot esculenta Crantz) somatic embryos,plantlets and tuberous roots[J].Proteome science,2010,8:10.
[4] 杨龙,张冠冬,宋雁超,等.野生和栽培木薯叶片的营养及饲料价值研究[J].南方农业学报,2017,48(2):238-245.
[5] 周璐丽,王定发,张振文,等.华南7号木薯茎叶营养价值评价[J].热带作物学报,2016,37(12):2245-2249.
[6] 冀凤杰,侯冠彧,张振文,等.木薯叶的营养价值、抗营养因子及其在生猪生产中的应用[J].热带作物学报,2015,36(7):1355-1360.
[7] MADEIRA A C,MENTIONS A,FERREIRA M E,et al.Relationship between spectroradiometric and chlorophyll measurements in green beans[J].Comm in Soil Sci and Plant Anal,2000,31(5-6):631-643.
[8] 惠伯棣. 类胡萝卜素化学与生物化学[M].北京:中国轻工出版社,2005.
[9] 康冬鸽,李瑞梅,胡新文,等.低温胁迫下木薯几种保护酶活性变化及其与耐寒性的关系[J].热带作物学报,2009,30(7):908-911.
[10] 黄秋凤,罗兴录,王春莲,等.低温胁迫对木薯幼苗生理特性的影响[J].中国农学通报,2010,26(17):172-177.
[11] 周玉飞,曾长英,陈新,等.低温驯化对木薯耐寒性形态、生理特性的影响[J].热带农业科学,2011,31(6):31-36.
[12] 吴海宁,罗兴录,樊吴静,等.低温胁迫对不同木薯品种幼苗生理特性的影响[J].南方农业学报,2013,44(11):1791-1799.
[13] 欧文军,罗秀芹,李开绵.低温胁迫对木薯组培苗叶片若干代谢生理指标的影响[J].热带作物学报,2015,36(4):706-712.
[14] LUO X Q,TOMLINS K I,LUIZ J C B C,et al. The analysis of vandidate genes and loci involved with carotenoid metabolism in cassava(Manihot esculenta Carntz) using SLAF-seq[J].Acta Physiologiae Plantarum,2018,40:66.
[15] 黄秋婵,许元明,韦友欢,等.深色叶植物叶片类胡萝卜素含量比较[J].湖北农业科学,2013,52(11):2573-2574.
[16] 陶俊,张上隆,徐昌杰,等.类胡萝卜素合成的相关基因及其基因工程[J].生物工程学报,2002,18(3):276-281.
[17] KANNANGARA R,MOTAWIA M S,HANSEN N K,et al.Characterization and expression profile of two UDP-glucosyl transferases,UGT85K4 and UGT85K5,catalyzing the last step in cyanogenic glucoside biosynthesis in cassava[J].Plant journal,2011,68(2):287-301.
[18] 肖亚冬,李大婧,刘春泉,等.叶黄素、β-隐黄质及其异构体检测方法的研究[J].现代食品科技,2016,32(2):335.
[19] 樊继鹏,徐振东,余文静,等.乳粉中叶黄素的测定方法及稳定性的影响[J].包装与食品机械,2014,3(24):20-23.
[20] 罗秀芹,杨龙,肖鑫辉,等.木薯块根类胡萝卜素积累与主要基因表达谱分析[J].福建农林大学学报,2018,47(2):138-143.

Analysis of carotenoids chlorophyⅡ and in cassava tissue culture seedlings

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