HUBEI AGRICULTURAL SCIENCES ›› 2019, Vol. 58 ›› Issue (24): 236-239.doi: 10.14088/j.cnki.issn0439-8114.2019.24.057

• Biological Engineering • Previous Articles     Next Articles

Adventitious buds induction of Vigna angularis and establishment of the regeneration system

LIN Jian-hui, LU Yu-jian, ZHANG Yong-lei, LI Zhen, ZHU Li-hui, SHI Dong-li   

  1. College of Biological and Environmental Engineering,Binzhou University/Shandong Provincial Engineering and Technology Research Center for Wild Plant Resources Development and Application of Yellow River Delta,Binzhou 256603,Shandong,China
  • Received:2018-10-20 Online:2019-12-25 Published:2019-12-25

Abstract: In this study, the hypocotyls were used as explants to analyze the factors that affected the adventitious bud induction and plant regeneration of Vigna angularis. An attempt was made to establish an efficient and stable regeneration system of Vigna angularis. The results showed that the optimal medium for the induction of adventitious buds was MS+2.0 mg/L 6-BA, and the optimum medium for rooting was 1/2MS+0.5 mg/L IBA. By analyzing the factors affecting the regeneration of Vigna angularis in vitro.

Key words: Vigna angularis, hypocotyl, adventitious bud, rooting, regeneration

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