HUBEI AGRICULTURAL SCIENCES ›› 2019, Vol. 58 ›› Issue (16): 139-141.doi: 10.14088/j.cnki.issn0439-8114.2019.16.032

• Biological Engineering • Previous Articles     Next Articles

Study on tissue culture technology of Dendrobium candidum Wall. ex Lindl. cv. Heijie

FENG Hai-dong1, JIN Shan-zhong1, ZHANG Bin2, ZHOU Ming1, ZHANG Ze-zhi1, ZHAO Zhi-quan3, GUO Rui2   

  1. 1.Shiyan Academy of Agricultural Sciences,Shiyan 442000,Hubei,China;
    2.Fangxian Municipal Bureau of Agriculture,Fangxian 442100,Hubei,China;
    3.Yanchihe of Danjiangkou City Traditional Chinese Medicine Cooperative,Danjiangkou 442000,Hubei,China
  • Received:2019-02-19 Online:2019-08-25 Published:2019-11-12

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Abstract: The protocorm induction and proliferation as well as seedling’ rooting of Dendrobium candidum Wall. ex Lindl. cv. Heijie stem tip were studied preliminarily. Dendrobium candidum Wall. ex Lindl. cv. Heijie stem tip was sterilized with 75% alcohol for 30 s, and then the epidermis was removed. The best protocorm induction and proliferation medium of Dendrobium candidum Wall. exLindl. cv. Heijie stem tip was 1/2 MS+5.5 g/L agar+0.55 mg/L 6-Benzylaminopurine+0.55 mg/L 1-Naphthalenea acid+0.1 mg/L AgNO3+100 g/L mashed potato+20 g/L white granulated sugar+0.45 g/L activated carbon. The best rooting medium of Dendrobium candidum Wall. exLindl. cv. Heijie seedling was 1/2 MS+5.5 g/L agar+0.15 mg/L 6-Benzylaminopurine+0.55 mg/L 1-Naphthalenea acid+0.1 mg/L AgNO3+100 g/L banana puree+20 g/L white granulated sugar+0.25 g/L activated carbon.

Key words: Dendrobium candidum Wall. Ex Lindl. cv. Heijie, stem tip, protocorm, proliferation, rooting

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