Abstract: With stem of Gynostemma pentaphyllum as explants, MS was used as the basic medium, and different plant growth additives were added to optimize the optimal medium for inducing callus, bud differentiation and rooting. Results showed that the optimal culture medium for callus proliferation was MS+2,4-D 2.0 mg/L +NAA 0.01 mg/L, callus volume increased 2.7 times; The most suitable bud differentiation medium was MS + 6-BA 2.0 mg/L + NAA 0.2 mg/L, the average number of bud 5.7; To take root the optimal medium for MS culture medium added 0.1 mg/L NAA, the rooting rate was 91%.

Key words: Gynostemma pentaphyllum, callus, proliferation, differentiation