Study on inducing callus of pear （Pyrus communis L. ‘Conference'） by orthogonal array-based method

doi:10.14088/j.cnki.issn0439-8114.2022.24.034

Abstract

Abstract: Pyrus communis L. ‘Conference' was used as material to induce callus, and L₉（3⁴） orthogonal design was used to explore the effects of various factors on callus induction of ‘Conference' pear.The results showed that the order of influence of each factor on callus induction： TDZ concentration > explants type > IBA concentration, and the influence of TDZ and IBA on the experimental results was significant （P<0.05）, in addition, there were significant differences among different levels. A₁B₃C₃ proved to be the best combination, stem segments were used as explants, MS+2.0 mg/L TDZ +0.5 mg/L IBA medium and dark culture for 35 days were adopted, and the induction rate was 100%.

Key words: Pyrus communis L. ‘Conference', tissue culture, callus, orthogonal design

CLC Number:

S661

YANG Li, WU Tao, LIU Zheng, CHENG Yin-sheng, LI Xie-yu, NIE Xian-shuang, QIN Zhong-qi. Study on inducing callus of pear （Pyrus communis L. ‘Conference'） by orthogonal array-based method[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(24): 160-162.

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