Tissue culture technology of stem segments of Tinospora sagittata

doi:10.14088/j.cnki.issn0439-8114.2021.15.030

Abstract

Abstract: Tinospora sagittate nodal segments in different positions were used as initial explants to explore the effect on axillary induction. Media were also screened for strengthening of weak plantlets. The results showed that positions of nodal segments significantly influenced the axillary induction and browning rate. With the positions of nodal segments from top to base of stems, the axillary induction rate decreased but browning rate increased gradually. The axillary induction rate of top no.1 nodal segment was 93.3%,which was significant higher than other positons of nodal segments. The browning rate of top no.1 nodal segment was 13.3% and the number of axillary buds （>0.5 cm） was 6.7. Maximum average length of shoots （4.1 cm） and average number of leaves （6.5） was recorded in the optimal strengthening media of MS+10 mL/L EDTA.

Key words: Tinospora sagittata, nodal segments, tissue culture, strengthening medium

ZHANG Xiao-yue, WEN Chao-ping, ZHOU Yu, DONG Kai-mi, CHEN Hua, WANG Ren-rui, HOU Da-bin, YANG Yu-xia, YU Ma. Tissue culture technology of stem segments of Tinospora sagittata[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(15): 144-147.

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