Abstract: Stems with buds in Ampelopsis grossedentata as test materials, culture medium and hormone combination in various stages in the process of cultivation of Ampelopsis grossedentata tissue and ratio of matrix in the seedling process were studied. Conclusions are as follows： The optimal primary medium in rapid propagation system of tissue cultured seedling of Ampelopsin Grossedentata was MS+1.5 mg/L 6-BA+0.2mg/L NAA+2 g/L PVP. The optimal matrix matching for primary stem cultured callus was MS+4.0 mg/L 6-BA+2.0 mg/L ZT+1.0 mg/L NAA. The optimal proliferation medium for Ampelopsin Grossedentata bud was MS+2.0 mg/L 6-BA+1.0 mg/L NAA+2.0 mg/L ZT. The optimal strong seedling medium was MS+1.0 mg/L 6-BA+1.0 mg/L NAA. The optimal rooting medium was NAA（0.1 mg/L）+IBA（0.5 mg/L）, with the rooting rate reaching 86.7%. The optimal transplantation matrix at hardening-seedling stage was peat + perlite （2:1）, and the hardening-seedling and transplanting survival rate could reach 73% if it was watered with modified Hoagland nutrient solution.

Key words: Ampelopsis grossedentata, tissue culture, rapid propagation system, medium, survival ratio