Effects of different media on anther culture of rice

doi:10.14088/j.cnki.issn0439-8114.2022.23.040

Abstract

Abstract: The effects of different basic media [N6, He5, SK3, M8 and modified M8 （GM8）] on callus induction efficiency of rice （Oryza sativa L.） with different indica and japonica components were studied to select the most suitable induction medium for rice anther culture. The results showed that the optimal induction medium was GM8; then GM8 was used as the basic medium, HB568 （japonica rice）, HB570 （glutinous rice）, HB613 （indica rice） and 21ah36 （wild rice） were used as experimental materials to compare the effects of single and compound growth regulators on rice anther culture. The results showed that the addition of compound growth regulators could improve the anther culture ability, and the best medium for rice anther culture was GM8+1.0 mg/L KT+2.0 mg/L 2,4-D+3.0 mg/L NAA.

Key words: rice（Oryza sativa L.）, anther culture, inducing medium, effectiveness of anther culture

CLC Number:

S511

ZHA Zhong-ping, GUO Ying, YIN De-suo, HU Jian-lin, ZHENG Xing-fei, DONG Hua-lin, LIU Yan-fang, WANG Hong-bo, XUE Lian, XU De-ze. Effects of different media on anther culture of rice[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(23): 202-205.

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