Identification and expression analysis of genes encoding carbohydrate metabolism enzymes and sugar transporters

doi:10.14088/j.cnki.issn0439-8114.2019.22.043

Abstract

Abstract: Starch accumulation in rice（Oryza sativa L.） seeds is the basis of rice yield formation. In filling stage sucrose is synthesized in rice leaves by photosynthesis, and then transported to seeds providing materials for starch synthesis. Several enzymes and transporters in this process have been reported. In this paper the genes encoding enzymes involved in carbohydrate metabolism including Calvin cycle, sucrose synthesis and degradation, starch synthesis and degradation and genes encoding sugar transporters were analyzed systematically. 148 genes encoding carbohydrate metabolism enzymes and 102 genes encoding sugar transporters were identified. 228 of these genes have been reported previously and 22 genes were found for the first time. Expression profile analysis indicates that some genes showed tissue specific expression pattern, which is closely related to their function. Expression of many genes was affected by abiotic stresses, demonstrating the important role of these genes in resistance to stress.

Key words: rice（Oryza sativa L.）, carbohydrate metabolism, sugar transporter, yield formation

CLC Number:

S511
Q78

WANG Yi-jie, ZHANG Shao-jie, LAI Yan, HU Yong-feng. Identification and expression analysis of genes encoding carbohydrate metabolism enzymes and sugar transporters[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(22): 185-193.

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