Study on tissue culture and rapid propagation of Ficushirta vahl

doi:10.14088/j.cnki.issn0439-8114.2021.01.032

Abstract

Abstract: To establish the system of tissue culture and rapid propagation of Ficushirta vahl.The seeds of Ficushirta vahl were used as experimental materials.The media of seed germination,adventitious bud proliferation and rooting were screened by conventional tissue culture technique.The result shows that 1/2 MS+sucrose 20 g/L+agar 6.0 g/L medium without any hormone was suitable for seed germination.The optimum medium of stem proliferation is MS+sucrose 20 g/L+agar 6.0 g/L.Tissue cultured plantlets root easily and the medium composition of 1/2 MS medium without any hormone sucrose 20 g/L+agar 6.0 g/L was good for rooting,The ratio of rooting of Ficushirta vahlcan reach 100%.The results can provide the theoretical and technical support for mass seedling production,germplasm resources preservation and breeding of Ficushirta vahl.

Key words: Ficushirta vahl, tissue culture, rapid prorogation technology

CLC Number:

CHEN Ying-zhi, HUO Xiu-yi, OUYANG Pu-yue, GAN Feng-qiong, LI Liang-bo, HUANG Rong-shao. Study on tissue culture and rapid propagation of Ficushirta vahl[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(1): 155-159.

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