Study on high efficient transformation of exogenous fatty acids into hydroxyfatty acids by engineering Escherichia coli

doi:10.14088/j.cnki.issn0439-8114.2020.01.035

Abstract

Abstract: Cytochrome P450BM3 can hydroxylates fatty acids into hydroxyfatty acids（HFAs） at the ω-positions with high entioselectivity. Based on the construction of Escherichia coli carrying P450BM3 gene to transform fatty acids. As an effort to solve inhibit fatty acid β-oxidation and increase outer membrane fatty acids transporter, the lipoacyl-coa synthase gene（fadD） deletion and outer membrane proteins encode gene（fadL） overexpression strains were built. Under shake-flask conditions, this recombinant strains produced 748.8 mg/L hydroxy dodecanoic acid, meanwhile the conversion rate was 93.6%.

Key words: lipoacyl-coa synthase gene（fadD）, outer membrane proteins encode gene（fadL）, hydroxy fatty acid, recombinant Escherichia coli

CLC Number:

TS206.4

WANG Xiang-wei. Study on high efficient transformation of exogenous fatty acids into hydroxyfatty acids by engineering Escherichia coli[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(1): 152-155.

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