Abstract: Three Chinese terrestrial orchids Cymbidium goeringii, Cymbidium tortisepalum Fukuy.var. longibracteatum and Cymbidium kanran were chosen to the samples for isolating and culturing rhizosphere fungi from substrates, which found and identificated three preponderant fungal strains during the storage, and which identified as Rhizopus oryaze, Chaetomium lobasum, Rhizopus icrosporus by 18SDNA sequences. It was found that the roots of orchid were mutualized with fungi to form rhizosphere structure, according to orchid roots stained and microscopical observed, so as to provide a theoretical basis for further research of orchids rhizosphere fungi.

Key words: orchid, rhizosphere fungi, separate, testing