HUBEI AGRICULTURAL SCIENCES ›› 2020, Vol. 59 ›› Issue (5): 88-91.doi: 10.14088/j.cnki.issn0439-8114.2020.05.017

• Horticulture & Local Products • Previous Articles     Next Articles

Analysis of amino acid metabolism in tobacco leaves among different varieties

LI Hong-chen1, ZHU Shun-cheng2   

  1. 1.Sanmenxia Company of Henan Tobacco Company,Sanmenxia 472000,Henan,China;
    2.Lushi Company of Sanmenxia Tobacco Company,Sanmenxia 472000,Henan,China
  • Revised:2019-09-23 Online:2020-03-10 Published:2020-06-05

Abstract: : To screen high-quality flue-cured tobacco varieties and clarify the differences in amino acid metabolism among flue-cured tobacco varieties, the dynamic changes of proline, glutamic acid and aspartic acid metabolism key enzyme activities and amino acid content during the tobacco leaf growth of Yuyan 10, NC89 and Zhongyan 100 were studied. The results showed that at 20 days of leaf age, the activities of Δ1-pyrroline-5-carboxylic acid synthetase, ornithine aminotransferase, proline dehydrogenase, aspartate aminotransferase and aspartate kinase all increased first and then decreased, the peak is at leaf age 30~40 d, Yuyan 10 had the highest enzyme activity; the content of proline in tobacco leaves increased continuously; after 30 days of leaf age, the content of proline in Yuyan 10 changed to highest. The content of free amino acids increased first, then decreased and then increased, and Yuyan 10 was the highest at each stage of leaf age.

Key words: flue-cured tobacco, tobacco leaf, amino acid, enzyme activity

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