Abstract: To establish a method to determine the contents of Cl^-,SO₄^2-,NO₃^-,PO₄^3- in animal skin gelatin and E jiao from different manufacturers by IEC. Results showed that Cl^-,SO₄^2-,NO₃^-,PO₄^3- were well separated and showed good linearity at 0.000 922~0.023 1, 0.001 09~0.027 3, 0.000 167~0.004 18, and 0.000 314~0.007 86 mg/mL. The average recoveries were between 98.7%~101.1%. Result of this method was accurate, sensitive, credible and repeatable. It could be applied to the content determination of 4 kinds of inorganic anions in animal skin gelatin and E jiao from different manufacturers.

Key words: E jiao, inorganic anionsion exchange chromatography, ion exchange chromatography（IEC）, content, determination