Abstract: HPLC method was used with acetonitrile∶0.1% formic acid as gradient elution. The detection wavelength was 254 nm, the flow rate was 1.0 mL/min, and the injection volume was 10 μL. At the same time, the contents of four components in Mesona chinensis Benth.,including caffeic acid, isoquercitrin, astragaloside and rosmarinic acid were determined, and the applicability of the system was investigated. The results showed that caffeic acid, isoquercitrin, astragaloside and rosmarinic acid had a good linear relationships in the concentration ranges of 34.297~171.480 μg/mL,11.172~55.860 μg/mL,16.386~81.928 μg/mL and 13.394~66.970 μg/mL, respectively. The correlation coefficients were 0.999 9, 1.000 0, 1.000 0 and 0.999 7, respectively. RSD of precision, repeatability and stability test results were all less than 3.00%. The average recoveries of the four components in the nine tests were all greater than 95.00% and RSD was less than 3.00%.

Key words: HPLC, Mesona chinensis Benth., content determination