Abstract: To establish the HPLC fingerprint of Strychni semen by TCM fingerprint technology, and provide a new method for the quality control of Strychni semen. The extraction methods was ultrasonic for 30 min by methanol-H₂O-HCl （50∶50∶1）. The mobile phase was acetonitrile-H₂O （0.2% formic acid）. The gradient elution mode was applied in chromatographic separation; the analysis time was 60 min; the detection wavelength was 254 nm; the column temperature was 30 ℃; the flow rate was 1.0 mL/min. The results showed that with brucine as the reference, six common peaks in the fingerprint of Strychni semen were determined. The RSD of precision, stability and reproducibility of the method was less than 3%. Using similarity as the evaluation parameter of fingerprint, the similarity between the chromatogram and the control fingerprint of 10 batches of Strychni semen was greater than 0.9.

Key words: Strychni semen, HPLC, fingerprint, quality control