Study on HPLC fingerprints of the aboveground and underground parts of Radix Ventilago Leicocarpa from different producing areas in Guangxi

doi:10.14088/j.cnki.issn0439-8114.2023.12.030

Abstract

Abstract: In order to establish HPLC fingerprints of the aboveground and underground parts of Radix Ventilago Leicocarpa, a comprehensive evaluation was conducted on the quality of the aboveground and underground parts of Radix Ventilago Leicocarpa from 10 production areas. Using Agilent ZORBX SB-C18 （4.6 mm×250 mm, 5 μm） Chromatographic column, acetonitrile 0.1% phosphoric acid gradient elution was conducted, with the flow rate of 0.8 mL/min, column temperature of 25 ℃, detection wavelength of 254 nm, and injection volume of 10 μL. The above-ground and underground parts of Radix Ventilago Leicocarpa from 10 production areas were evaluated through similarity evaluation, combined with cluster analysis and principal component analysis. This study had established HPLC fingerprints of the above-ground and underground parts of Radix Ventilago Leicocarpa and calibrated a total of 13 common peaks. According to the principal component comprehensive score model, a comprehensive evaluation was conducted on the above ground and underground parts of Radix Ventilago Leicocarpa from 10 production areas. The comprehensive quality of Radix Ventilago Leicocarpa in Pingle County, Guilin City, Guangxi was better. This method was easy to operate, had good repeatability, and fast analysis speed, and could be used for the comprehensive quality evaluation of Radix Ventilago Leicocarpa and to standardize the quality of Radix Ventilago Leicocarpa.

Key words: Radix Ventilago Leicocarpa, HPLC fingerprints, aboveground parts, underground parts, cluster analysis, principal component analysis, Guangxi

CLC Number:

R284

LIU Qian-qian, LI Wen-li, LIANG Xiao-lian, ZHOU Chu-hui, CHEN Yong, LUO Kong-xian, LIU Zhao-xiang. Study on HPLC fingerprints of the aboveground and underground parts of Radix Ventilago Leicocarpa from different producing areas in Guangxi[J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(12): 163-170,177.

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