Determination of N6-（2-hydroxyethyl）-adenosine in Paecilomyces cicadae by HPLC

doi:10.14088/j.cnki.issn0439-8114.2022.19.022

Abstract

Abstract: In order to establish a method for the determination of N6- （2-hydroxyethyl） -adenosine （HEA） in Paecilomyces cicadae 5704 s, HEA was extracted by ultrasonic assisted water bath method with deionized water as the solvent, and its content was determined by HPLC. The chromatographic conditions were optimized as follows： Agilent ZORBAX Extend-C₁₈ column （250 mm × 4.6 mm, 5 μm）; mobile phase was methanol ∶ 5 mmol/L ammonium acetate （containing 0.1% formic acid）; gradient elution; detection wavelength was 260 nm; flow rate was 1.0 mL/min; column temperature was 25 ℃; injection volume was 10 μL. The results showed that when the concentration of HEA was 0.25~100.00 μg/mL, the linear relationship between peak area and HEA content was good, R²= 0.999 9, the detection limit of the instrument was 0.25 μg/mL, the average recovery was 97.73%~108.95%, RSD was 0.37%~1.29%, and the content of HEA in the sample was 0.78 mg/g. The method is simple, rapid, accurate, stable and suitable for the determination of HEA in Paecilomyces cicadae 5704s mycelium.

Key words: Paecilomyces cicadae, N6-（2-hydroxyethyl）-adenosine（HEA）, HPLC, detection

CLC Number:

R284.2

LI Hui-wen, AI Ren-li, TAN Ai-juan. Determination of N6-（2-hydroxyethyl）-adenosine in Paecilomyces cicadae by HPLC[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(19): 114-118.

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