Study on HPLC fingerprint identification of Euphorbia maculata L. and its related species

doi:10.14088/j.cnki.issn0439-8114.2022.09.025

Abstract

Abstract: In order to establish and identify HPLC fingerprint analysis method of Euphorbia maculata L., PBONTOSIL KROMAPLUS 100-5-C18 column （250 mm × 4.6 mm, 5 μm） was used. Acetonitrile-0.1% phosphoric acid system was used as the mobile phase for gradient elution：Flow rate was 0.8 mL/min; detection wavelength was 360 nm; column temperature was 25 ℃. The results showed that there were significant differences in HPLC fingerprints between Euphorbia maculata L. and Euphorbia thymifolia L., Euphorbia prostrata Ait., Euphorbia taihsiensis （Chaw et Koutnik） Oudejians. The established HPLC fingerprint method can effectively identify Euphorbia maculata L. and its related species.

Key words: Euphorbia maculata L., related species, HPLC fingerprint, identification

CLC Number:

R284

HOU Jing, LI Li, JING Xiao-tong, LIANG Yuan, WEI Shan-shan, CAI Yi. Study on HPLC fingerprint identification of Euphorbia maculata L. and its related species[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(9): 127-131.

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