Abstract: Using thin layer chromatography （TLC）, using toluene： ethyl acetate： formic acid （10∶3∶1.5） as the developing agent, unfold, dry, spray 3% aluminum trichloride-ethanol solution, dry, and set the UV lamp （360 nm） view. Using high performance liquid chromatography, the column Eclipse XDB-C₁₈ （250 mm×4.6 mm, 5 μm）, 10 μL injection, acetonitrile-0.1% phosphoric acid aqueous solution as the mobile phase, gradient elution, flow rate was 1.0 mL/min, the temperature was 30 ℃, and the detection wavelength was 360 nm, establishing the thin-layer identification method and content determination method of Antenoron filiforme. Results showed that thin layer identification of Antenoron filiforme, spot cleaning, good separation, can effectively identify Antenoron filiforme. This method completed the analysis of the three flavonoid components within 30 minutes, and each component reached a baseline separation. The linear ranges of rutin, quercetin and kaempferol were 2.12~100.80 μg/mL（r=0.999 4）, 25.50~758.86 μg/mL（r=0.999 8）, 2.62~250.48 μg/mL （r=0.999 5）, respectively. The average recovery rates （n=6） were 98.42%, 101.21%, and 97.92%, respectively.

Key words: Antenoron filiforme, rutin, quercetin, kaempferol, thin layer identification, high performance liquid chromatography