Simultaneous determination of gallic acid,caffeine and catechins in tea by UPLC

doi:10.14088/j.cnki.issn0439-8114.2019.24.050

Abstract

Abstract: A qualitative and quantitative method for gallic acid, CAF and 8 catechins in tea were established by optimizing the extraction conditions of pretreatment and separation methods of Ultra Performance Liquid Chromatography（UPLC）. The results showed that the ACQUITY UPLC BEH C₁₈（1.7 μm,2.1 mm×50 mm） chromatographic column was used and the chromatographic conditions were as follows：The column temperature was 30 ℃, the flow rate was 0.2 mL/min, the mobile phase was 0.1% formic acid water -0.1% formic acid methanol solution, the detection wavelength was 278 nm. The 10 components in the range of 0.05~10.00 mg/L had a good linear relationship, the relative deviation of the content of each component in green tea samples RSD between 1.77%~5.89%. The method is simple, fast and accurate.

Key words: tea, ultra high performance liquid chromatography, catechins, caffeine, gallic acid

CLC Number:

ZHAO Ming-ming, ZHOU Cong, PENG Mao-min, ZHOU You-xiang. Simultaneous determination of gallic acid,caffeine and catechins in tea by UPLC[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(24): 205-208.

References 8

[1]	张静杰. 基于代谢谱分析的工夫红茶加工工艺优化及其品质形成研究[D].合肥:安徽农业大学,2013.
[2]	谭和平,邹燕,叶善蓉,等.茶叶中的多酚类物质及其分析方法综述[J].中国测试技术,2008(4):4-11.
[3]	吴迪,杨丽珠,仇佩虹,等.茶中儿茶素的分离分析方法研究进展[J].分析科学学报,2008,24(4):468-472.
[4]	谢果,何蓉蓉,栗原博.茶叶生物碱的生物合成与代谢的研究进展[J].中国天然药物,2010,8(2):153-160.
[5]	蔡明瑛,倪辉,李利君,等.液相色谱检测乌龙茶水浸提物及其单宁酶水解产物中的儿茶素[J].中国食品学报,2013,13(5):211-219.
[6]	吕远平,何强,姚开,等.儿茶素提取的优化条件研究[J].四川大学学报(工程科学版),2004,36(2):65-68.
[7]	吴娆. 食用植物油多元掺伪鉴别技术研究[D].北京:中国农业科学院,2016.
[8]	刘念. 电化学技术在茶叶分类及重金属含量检测中的探索[D].长沙:中南大学,2014.

[1]	ZHENG Ming-zhe. Research on the regional brand construction strategy of Ankang Se-enriched tea based on SWOT-CLPV model [J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(9): 180-184.
[2]	WANG Chun-hua, SUN Xue-fang. The function of astragalus polysaccharides in hepatic steatosis of mice [J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(8): 121-125.
[3]	ZHAO Yu-qing, ZHANG Xin, MU Kai-lang. Study on optimization of extraction process of volatile oil from mint produced in Anhui province [J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(8): 131-133.
[4]	ZHU Qiang, WANG Qian. The difficulties and countermeasures of the three rights division reform of homesteads from the perspective of rural revitalization [J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(7): 197-200.
[5]	HOU Yu-jing, QIAO Rui-huan, ZHAO Lin-long. Investigation and analysis on recognition of selenium-rich tea [J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(5): 162-166.
[6]	LIU Yu-ting, ZHANG Yu-ting, LAN Su-gui, CHEN Yu-quan, LI Li, TAN Qiang. Study on the enzyme expression and the washing performance based on the herbal saponin extracts as substrate [J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(3): 106-111.
[7]	LIU Bing-bing, LIU Jia, ZHANG Chen-ling, HAN Mei, JIA Na. Analysis of trace elements in dandelion tea extract and the dissolution characteristics by ICP spectroscopy [J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(3): 125-128.
[8]	WANG Ying, XIE Zhong-yuan, LI Feng, SHAO Huai-yong. Assessment of ecological vulnerability in the ecotone of the southeast margin of the Qinghai-Tibet plateau [J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(11): 39-44.
[9]	TIAN Hao. SWOT analysis of homestead reform：Taking Dali city as an example [J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(10): 171-174.
[10]	XIAO Jian, LIU Hui, ZHENG Fu-wei, LIU Song, QING Xiang-tao. Study on main meteorological and biochemical factors of quality formation of gold spring tea at different altitudes in Baojing [J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(1): 72-76.
[11]	CHEN Xu-ao, XUE Huan-xia, YAO Xiao-wei, ZHANG Lan. SWOT analysis and countermeasures for the improvement of quality and efficiency of Ankang selenium-enriched tea industry [J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(1): 165-168.
[12]	ZHOU Zhong-wen, LIU Ying, DU Jun, QIU Ning-gang, ZHANG Jun-lin, LI Qiao-xia, ZHANG Ke-xin. Analysis of apple climate quality certification in Xifeng Loess Plateau in 2018 [J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(8): 45-49.
[13]	HUANG Tao, FANG Meng-rui, XIA Hua-kun, ZUO Liang-liang, LYU Jun. Method of clustering segmentation for tea sprouts based on sharpness function [J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(8): 154-157.
[14]	JIE Wei-guang, YAO Yan-xuan, PENG Yong-zhen, HU Wei. Screening and identification of protease-producing bacteria from alkaline fermentation of excess sludge [J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(6): 143-147.
[15]	LI Cheng-bin, ZHANG Jun-song, YANG Shang-ying, LIU Yu-yu, YUAN Lin-cui, YANG Yan-mei, BAO Xiu-ping. Preparation of basil essential oils and its application in making cigarette [J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(6): 148-152.