Screening and identification of protease-producing bacteria from alkaline fermentation of excess sludge

doi:10.14088/j.cnki.issn0439-8114.2020.06.029

Abstract

Abstract: In order to shorten the alkaline fermentation period of excess sludge, alkaline-tolerant strains with high protease-producing activity were isolated from alkaline fermentation of excess sludge and identified by morphological. physiological, biochemical, and molecular characteristic analysis. The results showed that the two screened alkali-tolerant bacterial strains （HIT-01 and HIT-02 strains） which of high protease-producing activity were belongs to Bacillus subtilis and Bacillus sp. This study opens a new idea for further utilizing the screened dominant strains to construct microbial agents and quickly activate alkaline fermentation of sludge.

Key words: excess sludge, protease-producing bacteria, screening, identification

CLC Number:

中图法分类号：X703 文献标识码：A

JIE Wei-guang, YAO Yan-xuan, PENG Yong-zhen, HU Wei. Screening and identification of protease-producing bacteria from alkaline fermentation of excess sludge[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(6): 143-147.

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