Identification of two milk proteins of sow by mass spectrometry

doi:10.14088/j.cnki.issn0439-8114.2021.07.019

Abstract

Abstract: In order to identify several protein components of porcine milk, whole milk samples of large white pigs （n=8） were collected, and skim milk was obtained by centrifugation. SDS-PAGE was employed to separate skim milk proteins, and tree protein bands on SDS-gel were cut off, followed by treatments of reductive alkylation and trypsin digestion. The resulting peptides were loaded on LC-MS/MS to identify the three proteins with low molecular weight. Two proteins including β-lactoglobulin （β-LG） and whey acidic protein （WAP） were identified successfully. WAP may probably affect porcine milk functions.

Key words: sow, milk, protein identification, mass spectrometry

CLC Number:

S828

YUE An-qi, LUO Hui-hui, HUANG Wei-ping, CHEN Deng-quan, JIN Su-yu, HUANG Lin, ZHENG Yu-cai. Identification of two milk proteins of sow by mass spectrometry[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(7): 103-105.

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