HUBEI AGRICULTURAL SCIENCES ›› 2023, Vol. 62 ›› Issue (10): 172-177.doi: 10.14088/j.cnki.issn0439-8114.2023.10.030

• Detection Analysis • Previous Articles     Next Articles

Simultaneous determination of 10 nitroimidazole drugs residues in aquatic products by high-performance liquid chromatography-tandem mass spectrometry

XIE Yun-bo, YI Ming, TONG Wen-yu, ZENG Zhi, ZHU Zhi-qiang, SHI Yi-fu   

  1. Hubei Institute of Fisheries Science Research, Wuhan 430071, China
  • Received:2022-07-06 Published:2023-11-14

Abstract: A high-performance liquid chromatography-tandem mass spectrometry method was established for the detection of nitroimidazole drugs residues in aquatic products. The sample was extracted with ethyl acetate, distilled under reduced pressure, purified with n-hexane, and the residue was dissolved in 10% methanol before passing through a C18 chromatographic column (150 mm×2 mm, 5 μm). Separation was carried out using 0.20% formic acid aqueous solution acetonitrile as the mobile phase, gradient elution was performed, and the target compound was analyzed using high-performance liquid chromatography tandem mass spectrometry. The detection limit of the method was 0.04~0.30 μg/kg, the quantitative limit was 0.50~1.00 μg/kg, the linear relationship was good within the range of 1~150 μg/L, and the correlation coefficient (R2) was >0.998 00. At spiked level of 5.0, 7.5, 10.0, 15.0, and 20.0 μg/kg, the recovery rate was 73.0%~116.2%, and the relative standard deviation (RSD) was 0.43%~9.53%. This method was easy to operate, highly sensitive, and had good reproducibility, making it suitable for the detection of large batches of samples.

Key words: aquatic products, nitroimidazole drugs, high performance liquid chromatography tandem mass spectrometry, drugs residues

CLC Number: