Determination of biogenic amines in aquatic products by a new pre-column derivatization RP-HPLC method

doi:10.14088/j.cnki.issn0439-8114.2020.01.033

Abstract

Abstract: A new pre-column derivatization and reversed high performance liquid chromatography （RP-HPLC） was used to detect 8 biogenic amines in 15 kinds of aquatic products.,8 biogenic amines could be separated by a new precolumn derivatization method. The results showed that in the range of 1.0 to 100 mg/L, 8 biogenic amines showed a good linear correlation（R²≥0.998）, the detection limit and quantitative limit of the method were lower than that of the national standard method. The average recovery rate of the added standard was 90%~110%. The method was simple to operate,derivatives are stable, few impurity, accurate and reliable results, can be used to detect biological amines in fish and dried fish products.

Key words: aquatic products, biogenic amines, high performance liquid chromatography

CLC Number:

TS254.7

LI Su-yuan, YU Xi, HUANG Long, YU Jia. Determination of biogenic amines in aquatic products by a new pre-column derivatization RP-HPLC method[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(1): 145-148.

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