Simultaneous analysis of carbaryl and atrazine in water by HPLC with ultraviolet detection

doi:10.14088/j.cnki.issn0439-8114.2019.15.026

Abstract

Abstract: A method for simultaneous analysis of carbaryl and atrazine content in water was established by high performance liquid chromatography（HPLC） with ultraviolet detector. Samples were extracted and purified by ethyl acetate. The XDB-C18 column was used as chromatography column, and the mobile phase was a mixture of methanol-water（60∶40, V/V） at a flow rate of 1.0 mL/min. The column temperature kept 25 ℃, injection volume was 10.0 μL and the wavelength of detector was 221 nm. The results showed that within the range of 0.03~1.00 mg/L, it had good linear relationship between the peak area of carbaryl and atrazine and its mass concentration, and the linear correlation coefficient for carbaryl and atrazine was 0.999 9 and 0.999 9 respectively. The minimum detecion limits of quantitation of carbaryl and atrazine was 0.089 μg/L and 0.184 μg/L respectively. On addition level of 0.2~1.0 μg/L range, the recovery of carbaryl and atrazine for water was 85.12%~100.14% and 82.67%~105.51% respectively, and relative standard deviation（RSD） respectively was 1.60%~4.69% and 2.36%~4.26%. The method could be used for conventional analysis, which was simple, rapid, sensitive, accurate and with good precision.

Key words: carbaryl, atrazine, ultraviolet detection, high performance liquid chromatography

CLC Number:

ZHAO Wen-jin, GU Gui-fei. Simultaneous analysis of carbaryl and atrazine in water by HPLC with ultraviolet detection[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(15): 111-114.

References

[1] SOLOMON K R, GIESY J P, LAPOINT T W, et al.Ecologican risk assessment of atrazine in north American surface waters[J].Environ Toxicol Chem, 2013, 32(1):10-11.
[2] STOKCR T E, LAWS S C, GUIDICI D L, et al.The effects of atrazine on puberty in male wistar rats:An evaluation in the protocol for assessment of pubertal development and thyroid function[J].Toxicological sciences, 2000, 58(1):50-59.
[3] MUNGER R, ISACSON P, HU S, et al.Intrauterine growth retardation in low a communities with herbicide-Contaminated drinking water supplies[J].Environmental health perspectives, 1997, 105(3):308-314.
[4] SUSANNE W A.Sensitive enzyme immunoassay for the detection of atrazine based upon sheep, antibodies[J].Analytical letters, 1992, 25(6):1317-1408.
[5] ZHENG T Z, ZAHM S H, CANTOR K, et al.Agricultural exposure to carbamate pesticides and risk of non-hodgkin lymphoma[J].Journal of occupational & environmental medicine, 2001, 43:641-649.
[6] 胡洁, 王以燕, 许建宁.农药致癌性的研究进展[J].农药, 2009, 48(10):708-717.
[7] GB/T5750.9-2006, 生活饮用水标准检验方法农药指标[S].
[8] HJ 578-2010, 氧化沟活性污泥法污水处理工程技术规范[S].
[9] 安永生, 赵林林.在线固相萃取-高效液相色谱法同时测定水中甲萘威和阿特拉津[J].分析仪器, 2017(4):42-46.
[10] 万巧玲, 王良超, 余薇薇.UPLC-MS-MS同时快速检测水样中5种有机污染物[J].环境科学与技术, 2018, 41(S1):288-292.
[11] 张明, 唐访良, 徐建芬, 等.超高效液相色谱-串联质谱法快速测定地表水中联苯胺、苦味酸、甲萘威、阿特拉津和溴氰菊酯[J].色谱, 2018, 36(9):866-872.
[12] 王绍臣, 张剑平.超高效液相色谱-串联四级杆质谱法快速测定阿特拉津、甲萘威、微囊藻毒素LR、丙烯酰胺和联苯胺[J].环境科学与管理, 2016, 41(10):111-113.
[13] 许百环. 固相萃取-高效液相色谱法同时测定水中甲萘威、阿特拉津和苯并[a]芘[J].福建分析测试, 2017, 26(1):14-18.
[14] 王超, 吕怡兵, 腾恩江, 等.超高压液相色谱荧光检测法快速测定水中痕量苯胺与联苯胺[J].分析测试学报, 2013, 32(1):32-37.
[15] GB3838-2002, 地表水环境质量标准[S].
[16] 樊德方. 农药残留量分析与检测[M].上海:上海科技出版社, 1982.116-139.