Abstract: Develop a method for the determination of 2 kinds of sweeteners（saccharin sodium, aspartame） and 3 kinds of preservatives （benzoic acid, sorbic acid, dehydroacetic acid） in seasoning tea-based drinks by high performance liquid chromatography （HPLC）. A Diamonsil C₁₈ column （5 μm,250 mm×4.6 mm） was used with methanol -0.02 mol/L ammonium acetate （containing 0.4 mmol/L formic acid） solution as the mobile phase under the condition of gradient elution. Samples were extracted with water in an 50 ℃ ultrasonic bath for 30 min, and then they were analysed by VWD. Results show that the targets are baseline separated in 20 min. All correlation coef cients （r） are more than 0.999, with the average recoveries range from 83.0% to 108.0%, intra-day precision （RSD） values below 3.6% and inter-day precision （RSD） values below 4.4%. The method can be used for analyzing the targets in seasoning tea-based drinks.

Key words: seasoning tea-based drinks, high performance liquid chromatography, sweeteners, preservatives