HPLC analysis of sulforaphane in different kinds of leaf mustard （Brassica juncea Coss. var. foliosa Bailey） seeds

doi:10.14088/j.cnki.issn0439-8114.2020.17.035

Abstract

Abstract: In order to get the resources of leaf mustard with abundant sulforaphane,seeds of twenty cultivars were used to analyze and compare the contents of sulforaphaneby high performance liquid chromatography （HPLC）. The results showed that the range ofvariatoin of sulforaphane was 11.79~226.72 mg/kg（DW） with significant differences among tested lines. The material （Tai le sijiqing） with highest content of sulforaphane,which is 226.72 mg/kg （DW）,was about 19.2 times compared with the lowest one （Spring cobbage with round leaves） which is 11.79 mg/kg （DW）. It provides an foundational material for further research on rule of variation and the mechanism of metabolism of sulforaphan.

Key words: leaf mustard, sulforaphane, high performance liquid chromatography （HPLC）

CLC Number:

S637.2

LAN Mei, YANG Hong-li, HU Jing-feng, ZHANG Li-qin, XU Xue-zhong, HE Jiang-ming. HPLC analysis of sulforaphane in different kinds of leaf mustard （Brassica juncea Coss. var. foliosa Bailey） seeds[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(17): 154-157.

References

[1] 王萍,朱祝军. 不同采收季节对叶用芥菜黄酮物质含量和抗氧化活性的影响[J]. 园艺学报,2006,33(4):745-750.
[2] 郭彩杰,崔娜,李健,等. 十字花科植物中莱菔硫烷防癌机制研究进展[J]. 西北植物学报,2009,29(10):2146-2152.
[3] 梁浩,袁其朋,东惠茹,等. 十字花科植物种子中莱菔硫烷含量的比较[J]. 中国药学杂志,2004(12):18-19,29.
[4] SUN J, HUANG J.The effects of prevention of cancer bycruciferous vegetables[J]. Foreign Med Sci (Hyg),2003,30(1):19-23.
[5] CONAWAY C C,WANG C X,PITTMAN B,et al.Phenethyl isothiocyanate and sulforaphane and their N-acetylcysteine conjugates inhibit malignant progression of lung adenomas induced by tobacco carcinogens in A/J mice[J]. Cancer research,2005,65:8548-8557.
[6] YANAKA A, ZHANG S, TAUCHI R.Role of the Nrf 2 gene in protection and repair of gastric mucosa against oxidative stress[J]. Inflammopharmacology,2005, 13: 83-88.
[7] HU R,XU C,SHEN G, et al.Gene expression profiles induced by cancer chemopreventive isothiocyanate sulforaphane in the liver of C57BL/6J mice and C57BL/6J/Nrf2 (-/-) mice[J]. Cancer letter, 2006, 243(2): 170-192.
[8] KAMINSKI B M, LOITSCH S M, OCHS M J, et al.Isothiocyanate sulforaphane inhibits protooncogenic ornithine decarboxylase activity in colorectal cancer cells via induction of the TGF-beta/Smad signaling pathway[J]. Molecular nutrition & food research,2010,54: 1486-1496.
[9] FAHEY J W, HARISTOY X, DOLAN P M, et al.Sulforaphane inhibits extracellular, intracellular, and antibiotic-resistant strains of Helicobacter pylori and prevents benzo[a]pyrene-induced stomach tumors[J]. Proceedings of the national academy of sciences of the USA, 2002, 99: 7610-7615.
[10] ANNABI B,ROJAS-SUTTERLIN S,LAROCHE M,et al.The diet-derived sulforaphane inhibits matrix metalloproteinase -9-activated human brain microvascular endothelial cell migration and tubulogenesis[J].Molecular nutrition & food research,2008,52(6): 692-700.
[11] EVANS P C.The influence of sulforaphane on vascular health and its relevance to nutritional approaches to prevent cardiovascular disease[J]. The EPMA Journal, 2011, 2: 9-14.
[12] 李占省,刘玉梅,方智远,等. 芥蓝花薹中莱菔硫烷含量的HPLC分析[J]. 中国蔬菜,2016(4):53-57.
[13] 李占省,刘玉梅,方智远,等. UPLC-MS/MS快速测定甘蓝类蔬菜中活性成分莱菔硫烷[J]. 安徽农业科学,2015,43(36):1-3,6.
[14] FAHEY J W, ZALCMANN A T, TALALAY P.The chemical diversity and distribution of glucosinolates and isothiocyanates among plants[J]. Phytochemistry, 2001, 56(1):5-51.
[15] LIANG H,YUAN Q P, DONG H R, et al.Determination of sulforaphane in broccoli and cabbage by high-performance liquid chromatography[J]. J Food Compos Anal,2006,19(5):473-476.
[16] CAMPAS-BAYPOLI O N, SÁNCHEZ-MACHADO D I, BUENO-SOLANO C, et al. HPLC method validation for measurement of sulforaphane level in broccoli by-products[J]. Biomedical chromatography: BMC, 2010, 24(4):387-392.
[17] 胡晔,梁浩,袁其明. 十字花科植物种子中莱菔硫烷的薄层色谱鉴别[J]. 北京化工大学学报(自然科学版),2000,6(2):237-238.
[18] 何洪巨,唐晓伟,刘玲,等. HPLC-ESI/MS 鉴定十字花科植物中完形硫代葡萄糖甙[J]. 质谱学报,2003,24(3):385-388.
[19] 夏薇,赵秀娟,吴坤,等. 北方12种蔬菜中莱菔硫烷含量的测定[J]. 疾病控制杂志,2005,9(3):209-211.
[20] LIANG H,YUAN Q P,DONG H R,et al.Determination of sulforaphane in broccoli and cabbage by high-performance liquid chromatography[J]. J Food Compos Anal,2006,19(5):473-476.
[21] 张婵娟,郭晓玲,孟青,等. 不同品种西兰花种子中莱菔硫烷的HPLC分析[J]. 广东药学院学报,2007,23(5):505-508.
[22] 李占省,刘玉梅,方智远,等. 甘蓝和青花菜不同器官中莱菔硫烷含量差异研究[J]. 核农学报,2017,31(3):447-454.
[23] 王丽红,刘娟,王桂艳,等. 光果葶苈中莱菔硫烷的提取及含量测定[J]. 中国现代应用药学,2010,27(4):316-319.
[24] BROWN P D,TOKUHISA J G,REICHELT M,et al.Variation of glucosinolate accumulation among different organs and developmental stages of Arabidopsis thaliana[J]. Phytochemistry,2003, 62(3):471-481.
[25] IM J S,LEE E H,LEE J N,et al.Sulforaphane and total phenolics contents and antioxidant activity of radish according to genotype and cultivation location with different altitudes[J]. Korean journal of horticultural science & technology,2010,28(3):335-342
[26] FARNHAM M W,WILSON P E,STEPHENSON K K,et al.Genetic and environmental effects on glucosinolate content and chemoprotective potency of broccoli[J]. Plant breeding,2004,123(1):60-65.
[27] ABERCROMBIE J M,FARNHAM M W,RUSHING J W.Genetic combining ability of glucoraphanin level and other horticultural traits of broccol[J]. Euphytica,2005,143:145-151.
[28] 邱海荣,姚雪琴,谢祝捷,等. 反相高效液相色谱法检测青花菜和花椰菜中4-甲基亚磺酰丁基硫苷[J]. 上海农业学报,2008,24(4):72-74.
[29] 谢祝捷,李媛,姚雪琴,等. 青花菜基因型和环境互作对花球4-甲基亚磺酰丁基硫苷含量的影响[J]. 园艺学报,2010,37(4):625-630.