Determination of sugar and sugar alcohol content in plum extract by HPLC-ELSD

doi:10.14088/j.cnki.issn0439-8114.2023.12.029

Abstract

Abstract: A high performance liquid chromatography and evaporative light scattering detector （HPLC-ELSD） method was established for the simultaneous determination of fructose, glucose, sorbitol, sucrose, maltose hydrate, maltotriose, and maltotriose in plum extract. The separation was carried out using a Grace Prevail Carbohydrate ES column at a column temperature of 30 ℃; the mobile phase was acetonitrile water, with a flow rate of 1.00 mL/min;the detection used an evaporative light scattering detector, with a drift tube temperature of 60 ℃ and a nitrogen flow rate of 2.50 L/min. The results showed that fructose, sorbitol, glucose, sucrose, maltose hydrate, maltotriose, and maltotetraose had a good linear relationship,ranging from 34.69~2 220.00, 16.22~1 038.00, 38.66~2 474.00, 24.53~1 570.00, 22.91~1 466.00, 6.09~390.00 and 14.69~940.00 μg/mL,respectively,the precision was good, and the relative standard deviation （RSD） of repeatability was 1.00%~4.09%, the recovery rates of each target substance were 85.5%~110.1%, and the precision was 0.3%~4.6% （n=6）. This method achieved the separation of sugar and sugar alcohols within 30 minutes, with good sensitivity and repeatability detection, effectively avoiding the influence of solvents and temperature. The measurement results were accurate and the operation was easy, which could be used for batch detection of sugar and sugar alcohols in plum extract.

Key words: high performance liquid chromatography （HPLC）, evaporative light scattering detector （ELSD）, plum extract, sugar, sugar alcohol

CLC Number:

TS411

MA Shu-yi, LIANG Jia-xin, XU Chun-ping, ZHANG Ting, LI Ran, DONG Ai-jun, RONG Lin. Determination of sugar and sugar alcohol content in plum extract by HPLC-ELSD[J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(12): 158-162.

References

[1] 中国植物志编辑委员会. 中国植物志第三十八卷[M]. 北京:科学出版社,1986.
[2] GB2760—2014, 食品安全国家标准食品添加剂使用标准[S].
[3] 李紫云,杨美荣,张雁东. 核桃梅子酥性饼干的研制[J]. 粮食与食品工业,2016,23(1): 58-62.
[4] 马嫄,罗鸣,殷晓翠,等. 果梅的化学成分及应用研究进展[J]. 食品工业科技, 2018, 39(4): 337-341, 352.
[5] 林炎娟,方智振,周丹蓉,等. 基于网络药理学和分子对接技术研究乌梅改善消化不良的作用机制[J]. 食品安全质量检测学报,2022,13(7):2113-2121.
[6] 张华月,李琦,付晓伶. 乌梅化学成分及药理作用研究进展[J].上海中医药杂志, 2017, 51(S1): 296-300.
[7] 卢斌斌, 张启东, 肜霖, 等. 梅子提取物味觉特征成分的感官导向分析[J]. 河南工业大学学报(自然科学版), 2019, 40(1): 68-71,78.
[8] 凌志洲, 夏雨, 曾荣, 等.超声波辅助提取青梅有机酸及其成分分析[J].食品研究与开发,2020,41(12):140-146.
[9] 赵旭珠,夏陈,朱永清,等.不同地区不同成熟度青梅有机酸的测定及相关性分析[J].食品与机械, 2021, 37(11): 34-37, 43.
[10] 李江含. 乌梅质量评价及加工温度研究[D]. 成都:成都中医药大学, 2019.
[11] GB/T5009.7—2008,食品中还原糖的测定[S].
[12] 雷刚, 胡志辉, 陈禅友. 蒽酮比色定糖法实验条件的改进探讨[J]. 长江蔬菜, 2001(8): 38-39.
[13] 赵志明. 浅议《食品中葡萄糖的测定方法》及《食品中蔗糖的测定方法》[J]. 中国商检, 1997(12): 15.
[14] 杨柳, 王建立, 王淑英, 等. 糖类物质测定方法评价[J]. 北京农学院学报, 2009, 24(4): 68-71.
[15] 邓清平, 侯光菡. DPPH-HPLC-QTOF-MS/MS快速筛选和鉴定臭牡丹中抗氧化活性成分[J]. 华中师范大学学报(自然科学版), 2018, 52(6): 816-821.
[16] 胡婷, 邹成梅, 江吉周. 苦丁茶主物质提取及其HPLC指纹图谱分析[J]. 华中师范大学学报(自然科学版), 2021, 55(1): 61-71.
[17] 高国琰,董责章. 气相色谱法尿半乳糖醇及其他糖醇的测定[J].中国医科大学学报, 1995, 24(3): 247-249, 273.
[18] 杏艳, 潘春梅, 田渭花, 等. 气相色谱法测定固态酶解秸秆中糖类物质的含量[J]. 环境化学, 2012, 31(7): 1108-1109.
[19] 戴建英, 尤巍, 田甜, 等. HPLC-ELSD法同时测定知母药材中5种成分的含量[J]. 药学实践杂志, 2022, 40(1): 34-37.
[20] 吴国真, 王新茗, 王晓, 等. HPLC-ELSD法同时测定天麻中天麻素、葡萄糖、果糖和蔗糖含量[J]. 食品研究与开发, 2021, 42(20): 142-147.
[21] LI H, YANG Z Z, WANG J J, et al.Chemical fingerprint for identification and quality control of saccharides in danhong injection based on HPLC-ELSD with chemometrics[J]. Chemical research in Chinese universities, 2019, 35(5): 782-787.
[22] 傅徐阳, 鄂来明, 靳凤龙. 离子色谱法与液相色谱法测定乳制品中糖类物质的方法比较[J]. 中国乳品工业, 2020, 48(10): 57-59.
[23] 林慧, 颜春荣, 徐春祥, 等. HPLC-ELSD法同时测定食品中的10种糖和糖醇[J]. 食品科学, 2013, 34(12): 286-291.
[24] 曹邦静, 苏娟, 叶霁, 等. 蒸发光散射检测器在中药分析中的应用[J]. 药学实践杂志, 2015, 33(1): 13-16.
[25] 刘绍华,曲利利, 白家峰, 等.鸢尾根提取物膜分离组分分析及卷烟加香研究[J]. 云南大学学报(自然科学版), 2020, 42(6):1181-1189.
[26] 吴彦, 俞金伟, 马扩彦, 等. 芒果香味物质提取及其微胶囊在卷烟加香中的应用[J]. 香料香精化妆品,2018(4):6-11,16.
[27] 王慧, 蔡莉莉, 唐培培, 等. 枣子提取物中糖类含量的测定及提取工艺研究[J]. 烟草科技, 2014(5): 49-54.
[28] 吴立平, 曾辉, 孙咏梅, 等. 凝胶净化结合高效液相色谱-蒸发光散射检测器测定食用油脂中胆固醇含量[J].中国油脂, 2018, 43(6): 147-149.