Abstract: Malachite green, as a synthetic industrial dye of triphenylmethane, has been widely used in aquaculture as an insecticide and fungicide. GB/T20361—2006 for the detection of malachite green residues in aquatic products was found that had some disadvantages, such as tedious operation, poor recovery and precision during years of testing. The effects of different extraction times and purification times on the recovery rate, and improved the pretreatment and chromatographic conditions of GB/T20361—2006,reducing the number of extractions and purification operations reduces the flow rate of the chromatographic method. The improved method has a detection limit of 0.40 μg/kg, a recovery rate of 81.8%~100.8%, and a precision of 0.93%~8.32%, which all meet and exceed the national standard.

Key words: aquatic products, malachite green, HPLC