HUBEI AGRICULTURAL SCIENCES ›› 2023, Vol. 62 ›› Issue (10): 166-171.doi: 10.14088/j.cnki.issn0439-8114.2023.10.029

• Detection Analysis • Previous Articles     Next Articles

HPLC determination of quercetin and kaempferol in Qingqianliu leaves

LI Jun-xiua, LIAO Xia-yuna,b, ZHAO Li-chuna,b, LIANG Jiea,b   

  1. a. College of Pharmacy, Guangxi University of Chinese Medicine,Nanning 530200,China;
    b. Guangxi Zhuang Yao Medicine Engineering Technology Research Center, Guangxi University of Chinese Medicine,Nanning 530200,China
  • Received:2021-12-13 Published:2023-11-14

Abstract: The hydrolysis extraction conditions and content determination method of two flavonoid aglycones (quercetin and kaempferol) in Qingqianliu [Cyclocarya paliurus (Bata1.) Iljinskaja] leaves by high-performance liquid chromatography (HPLC) were studied to, provide reference for quality control of Qingqianliu leaves.Using ethanol-hydrochloric acid mixed solution as the solvent, the extraction and hydrolysis conditions were investigated using a combination of single factor and orthogonal experiments;content determination was conducted using Agilent C18 chromatographic column (250 mm × 4.6 mm, 5 μm) as the separation column and acetonitrile-0.1% formic acid aqueous solution (volume ratio 35∶65) as the mobile phase, with a detection wavelength of 370 nm, a column temperature of 30 ℃, and a flow rate of 1.0 mL/min. The results showed that the optimized extraction and hydrolysis conditions were as follows: Ethanol hydrochloric acid (volume ratio 8∶1) was used as the solvent, and the material-to-liquid ratio was 1∶20 each time, with a 90 ℃ constant temperature water bath reflux for 60 min. The injection volume of quercetin and kaempferol showed a good linear relationship, ranging from 0.080 8 to 0.808 7 μg (R2=0.999 7) and from 0.096 4 to 0.964 6 μg (R2=0.999 9), respectively;the average recovery rate of quercetin was 100.97%, with the relative standard deviation (RSD) of 2.93%. The average recovery rate of kaempferol was 97.25%, and the relative standard deviation was 3.29%. The extraction and hydrolysis process could significantly increase the content of flavonoid glycosides in Qingqianliu leaves;the established detection method was fast, accurate, and had good repeatability, which could provide technical reference for the quality evaluation of Qingqianliu leaves medicinal materials.

Key words: Qingqianliu [Cycloarya paliurus (Bata1.) Iljinskaja] leaves, HPLC, flavonoid aglycone, quercetin, kaempferol

CLC Number: