Abstract: The HPLC fingerprint of Magnolia officinalis flos was used to provide a new method for its quality control. Ultrasonic extraction with methanol for 30 min to prepare the test solution; Gradient elution was performed with methanol and water （0.1% formic acid） as mobile phases. The analysis time was 60 min. The detection wavelength was 230 nm. Column temperature was 30 ℃. The flow rate was 1.0 mL/min. Dozens batches of magnoliae officinalis flos were determined, and nine common peaks in the fingerprint of Magnoliae officinalis flos were determined with honokiol as the reference. The RSD of precision, stability and reproducibility tests were allless than 3% through methodological investigation. Using similarity as the evaluation parameter of fingerprint, the similarity between the chromatogram of Magnoliae officinalis flos samples and the control fingerprint was more than 0.9.

Key words: Magnoliae officinalis flos, HPLC, fingerprint, quality control