Exploring the utility of DNA barcoding in species identification of Chironomus

doi:10.14088/j.cnki.issn0439-8114.2021.05.028

Abstract

Abstract: The research analyzed 749 DNA barcodes collected the 12 species of Chironomus species and downloaded barcodes in BOLD and Genbank database by ABGD （Automatic Barcode Gap Discovery） and NJ （Neighbor-joining）. The result showed that ABGD analysed genetic distance, existed “DNA barcode gap”. The NJ tree supported 60 molecular operational taxonomic units, and keep conformed to morphological identification.In addition, based on the data are found, Chironomus sp.TE11 from public library should be Chironomus pseudothummi. Our result is an important supplement for the Chinese DNA barcodes study of Chironominae, and provide data support for establishing and perfecting the Chinese DNA barcodes database of Chironominae.

Key words: Chironomus, DNA barcoding, species identification

CLC Number:

Q969.44
Q523

YU Hai-jun, WANG Qian. Exploring the utility of DNA barcoding in species identification of Chironomus[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(5): 144-148.

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