Abstract: A strain of porcine pseudorabies virus：HuB2020 was isolated and purified from clinical materials, and a series of studies were carried out on its virulence, culture characteristics and genetic variation. The results showed thatthe nucleotide sequence of gE gene in this strain was 98.9%~99.9% with that of PRV （Pseudorabies virus,PRV） strain in China. TCID₅₀ of purified virus on PK15 cells was determined to be 10^7.5/mL. The LD₅₀ of 6-week-old Kunming mice was 1×10^5.7. Tissue sexual distribution showed that,10^6.0TCID₅₀/0.1 mL, 10^5.0 TCID₅₀/0.1 mL challenge groups showed 100% neurotropism to mice,and the detection rate of the virus in brain tissue and heart tissue was higher than other tissues.

Key words: porcine pseudorabies virus, separation, identification, genetic evolutionary analysis