Abstract: To establish and verify a HPLC method for the determination of lincomycin hydrochloride soluble powder. The determination was performed on a C₁₈ column （4.6 mm×150 mm,5 μm） at 214 nm with mobile phase of 0.02 mol/L diammonium hydrogen phosphate and acetonitrile （75∶25,V/V） at the flow rate of 1.0 mL/min. The column temperature was 30 ℃. The results showed that lincomycin showed a good linear relationship between the peak area and the concentration in the range of 0.1~4.0 mg/mL, the correlation coefficient was 0.999 7 （n=8）, the average recovery was 99.5%, RSD was 1.2%. The content of salt in the mobile phase was reduced, the pollution of salt solution to chromatographic column was reduced, the theoretical plate number of chromatographic peak was increased, and the mobile phase preparation was convenient and fast.

Key words: lincomycin hydrochloride soluble powder, content determination, high performance liquid chromatography