Isolation and identification of canine parvovirus CPV-JZ2021 strain and it's analysis of genetic evolution

doi:10.14088/j.cnki.issn0439-8114.2022.03.025

Abstract

Abstract: In order to study the biological characteristics, and genetic evolution of canine parvovirus, the feces of suspected dogs were collected from Hubei Xin-ai Pet Hospital Co. Ltd.. After sterility treatment, CRFK cells were inoculated with virus culture until obvious cytopathic changes appeared. The virus was verified by PCR, electron microscopy morphological observation, indirect immunofluorescence test and hemagglutination test. The results showed that a canine parvovirus was successfully isolated and named CPV-JZ2021. The hemagglutination titer of the virus is 2⁹, and the TCID₅₀ is 10^3.4/0.1 mL. Whole-genome sequencing showed that the length of the whole-genome sequence was 4 567 bp. The phylogenetic tree showed that the isolated strain was closely related to strains from Sichuan, Henan, Guangdong and Beijing, and belonged to the same evolutionary branch. The homology with CPV-HN1587 is 99.2%. The canine parvovirus belongs to the New CPV-2a type according to amino acid sequence of the VP2 gene. Compared with the New CPV-2a strains that have been reported abroad, CPV-JZ2021 has many key amino acid variations. This study provides a reference for the prevalence of canine parvovirus and the development of new vaccines.

Key words: canine parvovirus, isolation and identification, genetic evolution

CLC Number:

S855.3

LI Jian, WU Chu-jun, YE Shi-yi, CHEN Long-da, XIONG Tao. Isolation and identification of canine parvovirus CPV-JZ2021 strain and it's analysis of genetic evolution[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(3): 121-126.

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