Isolation and Identification of Antibacterial Bacteria in South China Sea

doi:10.14088/j.cnki.issn0439-8114.2018.10.015

Abstract

Abstract: The bacteria which had antibacterial activity was isolated from the sediments of South China Sea by plate isolation.The antibacterial activity of isolates were identified by paper disk method. The active isolates were identified by 16S rRNA sequencing and the phylogenetic tree constructed by neighbour-joining method. The growth characteristics of bacteria were studied. The results showed that nine active antibacterial bacteria belonging to genus Bacillus and Salinicola were isolated from sediment samples. It was indicated that strain j65 could grow at the concentration of 20% NaCl,and all strains could grow at the range of pH 6~10,except for strain gu8.

Key words: marine microorganism, isolation and identification, antibacterial experiment, phylogenetic tree

CLC Number:

Q939.99

TIAN Liang-yu, PENG Shu-ya, GU Hui-hui, JIANG Chao, ZHU Dao-chen, ZHOU Xiao-jian. Isolation and Identification of Antibacterial Bacteria in South China Sea[J]. HUBEI AGRICULTURAL SCIENCES, 2018, 57(10): 65-68.

References

[1] 薛超波,王国良,金珊,等.海洋微生物多样性研究进展[J].海洋科学进展,2004,22(3):377-384.
[2] 胡申才. 具细胞毒活性海洋微生物的分离筛选及次级代谢物的研究[D].武汉:华中农业大学,2005.
[3] 梁静娟,詹萍,庞宗文.具有抗真菌活性的海洋微生物的分离筛选[J].现代食品科技,2006,22(2):92-94.
[4] 王幸,吴文惠,陈志华,等.海洋微生物次生代谢产物的结构特征和生物活性的研究进展[J].中国天然药物,2010,8(4):309-320.
[5] 何建瑜,赵荣涛,陈永妍,等.海洋微生物多样性研究技术进展[J].生命科学,2012,24(6):526-530.
[6] 姜键,杨宝灵,元起,等.具有抑菌活性的海洋细菌的分离与鉴定[J].生物技术,2004,14(6):28-30.
[7] 缪莉,郑忠辉,苏文金.共附生海洋微生物活性物质的研究进展[J].海洋通报,2002,21(3):62-68.
[8] RUBIO R,JOFRÉ A,MARTÍN B,et al. Characterization of lactic acid bacteria isolated from infantfaeces as potential probiotic starter cultures for fermented sausages[J].Food Microbiology,2014,38(4):303-311.
[9] ELISASHVILI V,KACHLISHVILI E.Physiological regulation of laccase and manganese peroxidase production by white-rot Basidiomycetes[J].Journal of Biotechnology,2009,144(1):37-42.
[10] 郭娜燕,连莲香,黄耀坚,等.福建沿海沉积物放线菌的多样性及生物活性[J].中国抗生素杂志,2017,42(1):20-27.
[11] 周德庆. 微生物学教程[M].第三版.北京:高等教育出版社,2011.
[12] 康白. 微生态学[M].第三版.辽宁大连:大连出版社,1988.
[13] 沈萍. 微生物学[M].北京:高等教育出版社,2000.
[14] HAHN M G.Microbial elicitors and their receptors in plants[J].Annual Review of Phytopathology,1996,34:387-412.
[15] 向亚萍,陈志谊,罗楚平,等.芽孢杆菌的抑菌活性与其产脂肽类抗生素的相关性[J].中国农业科学,2015,48(20):4064-4076.
[16] 黄秀梨. 微生物学[M].北京:高等教育出版社,1998.
[17] 高增贵,陈捷,高洪敏,等.玉米茎腐病菌产生的细胞壁降解酶种类及其活性分析[J].植物病理学报,2000,30(2):148-152.
[18] 朱飞舟,陈利玉,陈汉春.16S rRNA基因序列分析法鉴定病原细菌[J].中南大学学报(医学版),2013,38(10):1035-1041.
[19] 李聪,蔡岩,周永灿,等.海南罗非鱼致病性维氏气单胞菌分离鉴定及药敏特性研究[J].水产科学,2015,34(10):640-646.
[20] ZHU D,TANABE S H,XIE C X,et al.Bacterial community composition of South China Sea sediments through pyrosequencing-based analysis of 16S rRNA genes[J].PloS One,2013, 8(10):e78501.
[21] 林凤敏,姬文秀,尹祯焅,等.长白山天池水可培养细菌的16S rRNA鉴定[J].延边大学农学学报,2010,32(2):77-81.
[22] ZHU D C,TANABE S H,XIE C X,et al.Bacillus ligniniphilus sp. nov. an alkaliphilic and halotolerant bacterium isolated from sediments of the South China Sea[J].International Journal of Systematic & Evolutionary Microbiology,2014,64:1712-1717.