Isolation and identification of HB201602 strain of porcine epidemic diarrhea virus and phylogenetic tree analyses based on S1 gene

doi:10.14088/j.cnki.issn0439-8114.2019.24.055

Abstract

Abstract: Porcine epidemic diarrhea virus （PEDV） HB201602 strain was isolated from the piglets suspected to be suffering a severe diarrhea in Hubei Province. The isolated strain can infect Vero cells and cause obvious cell pathological change, with viral titer up to 1.0×10^5.6 TCID₅₀/0.1ml. Indirect immunofluorescence results indicated that HB201602 infected cells could be recognized by monoclonal antibodies against PEDV S protein, further confirming that the isolated strain was PEDV virus. Phylogenetic trees were constructed based on S1 gene, and the results indicated that most of the strains isolated after 2011 were G2 variant strains, and HB201602 belonged to G2-a subtype （variant strain） cluster. Genetic variation analysis of S1 gene shows that G1-b subtype strain has 9 specific single amino acid mutations compared with other cluster strains, and G1-a subtype classical strain has 4 specific amino acid mutations. Variation analysis of S1 gene will provide a basis for understanding the genetic variation trend of PEDV and the development of new vaccines.

Key words: porcine epidemic diarrhea virus, isolation, sequence analysis, phylogenetic tree, genetic variation

CLC Number:

S852.65⁺1

LIU Wei, YUAN Fang-yan, ZHOU Dan-na, LIU Ze-wen, YANG Ke-li, DUAN Zheng-ying, GUO Rui, GAO Ting, LIANG Wan, TIAN Yong-xiang. Isolation and identification of HB201602 strain of porcine epidemic diarrhea virus and phylogenetic tree analyses based on S1 gene[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(24): 227-231.

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