Identification and genome sequence analysis of pepper mild mottle virus isolate from Paris polyphylla var. yunnanensis

doi:10.14088/j.cnki.issn0439-8114.2020.13.035

Abstract

Abstract: Paris polyphylla var. yunnanensis is a perennial herb, and its medicinal part is underground rhizomes. It is an important chinese medicinal material. In order to clarify the viral pathogens infecting the Paris polyphylla, the transmission electron microscopy, DAS-ELISA and RT-PCR techniques were performed on the Paris polyphylla collected from Qujing, Yunnan province. Baculovirus particles were observed under transmission electron microscopy and were（200~300）nm ×（20~36）nm in size. The results of DAS-ELISA on 7 samples collected showed that 5 of them were positive. A whole genome sequence of a 6 357 bp Qujing isolate （PMMoV-QJ, accession number MK784568） was cloned by RT-PCR amplification. The homologous sequence of PMMoV-QJ were analyzed by homology with 10 PMMoV isolates reported at home and abroad, and the homology was between 99.42% and 99.81%. Phylogenetic analysis based on the whole gene sequence showed that PMMoV-QJ was closely related to Korean isolates. This study clarifies the genetic relationship of the isolate, and identifies the isolate from the molecular level, which provides a theoretical basis for the prevention of the subsequent viral disease.

Key words: Paris polyphylla var. yunnanensis, pepper mild mottle virus（PMMoV）, identification, sequence analysis

CLC Number:

S432.4⁺1

YANG Lin-yi, CHEN Lu, CHEN Ze-li, FEI Jin-qiang, WANG Zhe, XU Shao-zhong, ZHAO Ming-fu, WEN Guo-song. Identification and genome sequence analysis of pepper mild mottle virus isolate from Paris polyphylla var. yunnanensis[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(13): 154-157.

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