HUBEI AGRICULTURAL SCIENCES ›› 2020, Vol. 59 ›› Issue (11): 131-133.doi: 10.14088/j.cnki.issn0439-8114.2020.11.027

• Biological Engineering • Previous Articles     Next Articles

Cloning and sequences analysis of matK gene of Amorphophallus konjac from Ankang city

ZHOU Xiao-fan, LI Jing, WANG Rui, LI Rui, WANG Rui-xian   

  1. College of Modern Agriculture and Biotechnology, Ankang University, Ankang 725000, Shaanxi, China
  • Received:2019-08-09 Online:2020-06-10 Published:2020-08-06

Abstract: The genome of the Amorphophallus konjac from Ankang city was extracted and the matK gene sequence was amplified. After confirmed by gel electrophoresis, amplified product was sequenced and analyzed. Results showed that the matK gene of Amorphophallus konjac from Ankang city was 1 551 bp in length and predicted to encode 516 amino acids. The phylogenetic tree showed that the Amorphophallus konjac had the closest relationship with Amorphophallus maxwellii, Amorphophallus corrugatus and Amorphophallus krausei.

Key words: Amorphophallus konjac, matK gene, sequence analysis, Ankang city

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