Study on the isolation, identification and drug resistance of main pathogenic bacteria in dairy cow mastitis

doi:10.14088/j.cnki.issn0439-8114.2019.21.031

Abstract

Abstract: In order to understand the microflora distribution and drug resistance of the pathogenic bacteria of cow mastitis, a one-year pathogen isolation and identification and drug resistance study was conducted in a dairy farm in Hubei Province. The results showed that 235 strains of pathogens belonging to16 genera of 9 families were isolated from 165 milk samples. Among them, 83 strains were Staphylococcus, accounting for 35%; 36 strains were Protothecazopfii, accounting for 13.5%; 28 strains were Acinetobacterrubrum, accounting for 11.9%; 12 strains were Escherichia coli, accounting for 5.1%; 7 strains were Streptococcusdysgalactiae, accounting for 2.9%; and other strains accounting for 31.6%. The results of drug resistance test showed that 20 isolates had different degrees of drug resistance to the 14 antibiotics tested. The pathogen is resistant to at least one drug, and the highest is resistant to 14 drugs. The most sensitive antibiotic is florfenicol, with a sensitive rate of 75%. The results provide a fundamental data for the prevention and control the dairy cow mastitis and clinical medication.

Key words: cow mastitis, pathogenic bacteria, isolation and identification, drug resistance

CLC Number:

S857.2⁺6

WU Li-jun, LI Jia-lin, CHEN Xia-bing, WAN Ping-min, SHAO Zhi-yong, HE Bin, YANG Wen-hai, CHEN Jie, JIN Er-guang, PAN Zhong-bao, TONG Wei-wen, ZHOU Yuan, LIU Wu. Study on the isolation, identification and drug resistance of main pathogenic bacteria in dairy cow mastitis[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(21): 145-149.

References

[1] 杜琳,王丽芳,冯小慧,等.我国部分地区奶牛乳房炎主要病原菌的分离鉴定及药敏试验[J].动物医学进展,2018,39(3):124-128.
[2] MUNQUBE E O,TENHAGEN B A,REQASSA F,et al.Reduced milk production in udder quarters with subclinical mastitis and associated economic losses in crossbred dairy cows in Ethiopia[J].Trop Anim Health Prod,2005,37(6):503-512.
[3] 阿继春,毛成荣.奶牛乳房炎及其防治[J].山东畜牧兽医,2016, 37(1):19-20.
[4] 蒋春茂,周新民,周广生,等.奶牛乳房炎研究进展[J].中国畜牧兽医,2004,31(5):36-38.
[5] CHANETON L,TIRANTE L,MAITO J,et al.Relationship between milk lactoferrin and etiological agent in the mastitic bovine mammary gland[J].J Dairy Sci,2008,91(5):1865-1873.
[6] 李新圃,李宏胜,罗金印,等.奶牛乳房炎致病菌的分离鉴定及耐药性研究[J].动物医学进展,2015,36(11):36-39.
[7] COSTA E O,CARCIOFI A C,MELVILLE P A,et al.Prototheca sp. outbreak of bovine mastitis[J].Journal of veterinary medicine series B,1996,43(6):321-324.
[8] ROESLER U,HENSEL A.Longitudinal analysis of Prototheca zopfii specific immune responses:Correlation with disease progression and carriage in dairy cows[J].J Clin Microbiol,2003, 41(3):1181-1186.
[9] 张寒琪,高健,吴桥兴,等.奶牛中型无绿藻性乳房炎[J].中国兽医杂志,2011,47(5):13-15.
[10] 戴迪,廖俊,林伟东,等.4株奶牛源左氏无绿藻的分离与鉴定[J].安徽农业科学,2018,46(1):78-79,86.
[11] 刘朝,王京仁,张成栋,等.湖北地区奶牛乳房炎病原菌的分离鉴定与耐药性分析[J].中国奶牛,2007(7):35-38.
[12] 高原,张博,姚伟.奶牛乳房炎实验室诊断技术研究进展[J].现代畜牧兽医,2018(4):54-59.
[13] 叶平,刘开永.奶牛乳房炎的治疗[J].中国奶牛,2003(5):43-45.