Isolation,Identification and Antimicrobial Traits of Antibiotic Resistant Strains in Environment

doi:10.14088/j.cnki.issn0439-8114.2018.10.014

Abstract

Abstract: By using Gram stain, physiological property and phylogenetic trees based on 16S rDNA,the different antibiotic resistant strains which were screened and isolated from soil were identified. Then the growth chart of strains were measured,and the drug resistance and the antimicrobial characteriatics were detected. The results showed that 11 antibiotic resistant strains were isolated and identified from soil. According to morphological characteristics and the phylogenetical analysis of 16S rDNA,these strains were determined to belong to Pseudomonas, Variovorax, Lysobacte and Brevibacillus. And the strains of XC-10,XN-4,XN-5 and XN-6 had different inhibitory effects on the growth of bacteria such as Staphylococcus aureus,Bacillus subtilis,and Micrococcus luteus. Screened had obvious inhibiting effect on six common indicator strains. The 11 strains all showed significant drug-resistance to the final concentration of 10 μg/mL spectinomycin and 20 μg/mL ampicillin.

Key words: antibiotic resistant strains, isolation and identification, 16S rDNA, antimicrobial characteriatics

CLC Number:

Q93-331

YAN Chun-lan, YANG-QING Zhuo-ma, WEI Ying-mei, WANG Tao, LEI Shi-ting, WANG Lei. Isolation,Identification and Antimicrobial Traits of Antibiotic Resistant Strains in Environment[J]. HUBEI AGRICULTURAL SCIENCES, 2018, 57(10): 60-64.

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