Isolation and Identification of Antagonistic Fungus MA4 against Fusarium oxysporum f. sp. tracheiphilum Causing Cowpea Wilt Disease

doi:10.14088/j.cnki.issn0439-8114.2019.02.018

Abstract

Abstract: ： To screen microbial resources for biological control of cowpea wilt, a dominant antagonistic fungus MA4 strain against Fusarium oxysporum f. sp. tracheiphilum was isolated, purified and screened from rhizosphere soil in Wuhan by dilution plating and confront culture method. The MA4 strain was identified according to its morphology and 18S rDNA sequence. The antifungal spectrum and stability of MA4 strain were studied by the plate confrontation method, and the pot experiment was also carried out. The results showed that the antifungal activity of MA4 against F. oxysporum f. sp. tracheiphilum was 75.69%. According to its morphology and 18S rDNA sequence analysis, the MA4 strain was identified as Trichoderma harzianum, and registered the sequence in GenBank with a login number of MH539514. The antifungal activity of MA4 against Botrytis cinerea, F. oxysporum Schlecht, F. oxyporum f. sp. niveum, F. oxysporum. f. sp. cucumebrium, Phomopsis vexans, Alternaria solani, P. vexans and Rhizoctonia solani was from 62.12% to 86.93%. After the subculture of 10 generations, the antifungal activity of MA4 against F. oxysporum f. sp. tracheiphilum was 80.86%. The results of the potted experiment showed that the disease index of the treatment with MA4 fermentation liquor after 14 d inoculation was 11.85%, the prevention efficiency was 85.75%, and it had certain promoting effect on cowpea plants.

Key words: Fusarium oxsyporum schl. f. sp. tracheiphlium, isolation and identification, antagonistic fungus, Trichoderma harzianum

CLC Number:

S436.43

SHEN Jun, YANG Shao-li, WU Ren-feng. Isolation and Identification of Antagonistic Fungus MA4 against Fusarium oxysporum f. sp. tracheiphilum Causing Cowpea Wilt Disease[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(2): 79-83.

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