Cloning and genetic evolution analysis of one strain of newcastle disease virus

doi:10.14088/j.cnki.issn0439-8114.2019.15.031

Abstract

Abstract: A Newcastle disease virus（NDV） was isolated from ND suspected chickens of a scale chicken farm in Xinxiang. The F gene of the isolated Newcastle disease virus（NDV） was amplified and analyzed by genetic evolution. The results showed that the whole nucleotied sequence length of F gene was 1 662 bp. The sequence of amino acid of F gene at the cleavage site is 112R-R-Q-K-R-F117, which is consistent with the characteristics of strong NDV strain. Comparison of the NDV with the strong NDV of gene Ⅶ of epidemic strains from different countries, results showed that the nucleotide homology was 87.5%~97.7%, and in which the comparison with the Ⅶd was 95.4%~97.7%. Comparison of the NDV isolated with the current vaccine strains such as La Sota, Clone-30, B1, Mukteswar and so on, the nucleotide homology was 84.2%~86.6%. The phylogenetic tree analysis demonstrated that the NDV isolated and Ⅶd strains of FJ882014, FJ608335 and GU227738 were located in the same cladogram branch.

Key words: Newcastle disease virus, F gene, cloning, sequence analysis, genetic evolution, chicken

CLC Number:

XU Ping, XU Xiao-bo, TATIANA Fotina, WANG San-hu, ZHAO Kun. Cloning and genetic evolution analysis of one strain of newcastle disease virus[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(15): 132-135.

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