Antitumor effect of EPF monoclonal antibody combined with cisplatine on animal bladder cancer in livestock：Based on a bladder tumor model in nude mice

doi:10.14088/j.cnki.issn0439-8114.2020.09.028

Abstract

Abstract: To investigate the antitumor effects of early-pregnancy-factor monoclonal antibody （EPF-McAb） combined with cisplatin（DDP） on human bladder cancer EJ cell in nude mice, and to explore its possible mechanism, a melanin xenograft model of nude mice was established. When the diameter of the transplanted tumor grew to 5mm, the mice were randomly divided into 4 groups, a blank group with no treatment, a control group treated with cisplatin, and two treatment group treated with low dosage EPF-McAb combination and high dosage EPF-McAb combination, respectively. The volume of transplanted tumor was measured every 2 days after the administration tp plot the tumor growth curve. On the 10th day after the last administration, the tumors were taken and weighed to calculate the tumor inhibition rate. The pathology changes of carcinoma were observed after HE staining by light microscope. The expression of EPF protein was detected by Western blot. The results showed that the growth rate of the transplanted tumors in the two treat groups were significantly slower than that in the blank group and the control group （P<0.05）. The tumor inhibition rate of the transplanted tumors in the low-dose EPF-McAb group and high-dose EPF-McAb was 35.78% and 53.04%, respectively, which were both significantly higher than that in the control group （with tumor inhibition rate of 22.36%）（P<0.05）. And the expression of EPF protein in treatment groups was also decreased. It indicated that EPF-McAb combined with DDP can effectively inhibit the proliferation of bladder cancer EJ cells in nude mice. The mechanism may be related to down-regulation of EPF protein expression.

Key words: early pregnancy factor monoclonal antibody（EPF-McAb）, cisplatin, bladder cancer, livestock

CLC Number:

R739.5

ZHONG Yong-yi, ZHOU Quan, SU Li-jie, LIAN Yu-yin, WANG Jia-ji, CHEN Xiao-yi. Antitumor effect of EPF monoclonal antibody combined with cisplatine on animal bladder cancer in livestock：Based on a bladder tumor model in nude mice[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(9): 133-136.

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