HUBEI AGRICULTURAL SCIENCES ›› 2020, Vol. 59 ›› Issue (17): 97-100.doi: 10.14088/j.cnki.issn0439-8114.2020.17.023

• Aquatic Products • Previous Articles     Next Articles

Study on phages in control of common bacterial diseases in Macrobrachium nipponense and its application

HANG Xiao-ying1, YU Zhe1, LYU Sun-jian1, SHI Wei-da1, YUAN Xue-mei1, LIU Li2   

  1. 1. Zhejiang Institute of Freshwater Fisheries/Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture/Key Laboratory of Fish Health and Nutrition of Zhejiang Province, Huzhou 313001, Zhejiang, China;
    2. Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
  • Received:2020-05-22 Published:2020-10-23

Abstract: 41 bacterial strains were isolated from water and shrimps of three Macrobrachium nipponense ponds, by 16S rDNA gene sequence analysis and colony morphology observation, the strains from water were preliminarily identified as Bacillus megaterium, Aeromonas caviae, Enterobacter roggenkampii,et al, the strains from shrimp were preliminarily identified as Aeromonas caviae, Citrobacter murliniae, Enterobacter roggenkampii, et al. The phage NTHP01 inhibition of bacteria was detected by drop injection methodthe results showed that, phage NTHP01 had good cracking effect on Bacillus, Aeromonas, Acinetobacter, Enterobacter, Citrobacter, Arthrobacter protophormiae, Orthomonad, Lactococcus garvieae, et al. Phage NTHP01 preparation was appliled in shrimp breeding pond, the preliminary results showed that, the yield of Macrobrachium nipponense increased by 338~412 kg/hm2, and the output value increased by 2.25 ten thousand ~3.00 ten thousand yuan/hm2.

Key words: phage, Macrobrachium nipponense, bacterial diseases, control

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