Effects of pioglitazone on oocytes maturation in vitro and parthenogenetic embryos development of pig

doi:10.14088/j.cnki.issn0439-8114.2020.17.022

Abstract

Abstract: Aiming to investigate the effects of pioglitazone（PIO） on oocytesmaturation in vitro and parthenogenetic embryos development of pig, the effect of different concentration PIO on oocytes maturation in vitro and parthenogenetic embryos developmental potency were compared. The results showed that the polar body excretion rate of 1.0 μmol/L PIO-treated group was significantly higher than that of 0.5 μmol/L PIO-treated group in maturation medium（P<0.05）,was very significantly higher that of control an 1.5 μmol/L PIO-treated group（P<0.01）. The mature oocytes cultured in different concentrations PIO were parthenogenetic activated, the experimental results showed that the cleavage rate of the 1.0 μmol/L PIO group was significantly higher than of the 0.5 μmol/L group in embryo culture medium（P<0.05）, was very significantly higher than that of the control and 1.5 μmol/L PIO group （P<0.01）, as for the blastocyst rate and the total number of cells, the group of the 1.0 μmol/L PIO-treated group was the highest among the groups （P<0.01）. In conclusion, 1.0 μmol/L PIO in maturation medium could improve the maturation rate of porcine oocyte, also could significantly improve the blastocyst rate and the total number of cells of porcine parthenogenetic embryos.

Key words: pioglitazone, pig, oocytes, parthenogenetic embryos, developmental effect

CLC Number:

S828
Q954.4

LYU Ling-yan, WANG Yan-ling, PAN Xing-chen, ZHANG Jia-qing, WU Yong-shao, SUN Ru-yu, PAN Tian-biao, ZHANG Bing. Effects of pioglitazone on oocytes maturation in vitro and parthenogenetic embryos development of pig[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(17): 93-96.

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